Quantitative Biology of Membrane Traffic and Pathogenesis


Our team aims at understanding the molecular mechanisms that control the spatiotemporal organization of endocytic proteins and cell architecture, which has a fundamental biologic relevance and might be key to identify new therapeutic targets.

Endocytosis is a fundamental cellular process that is dedicated to nutrient uptake and to the regulation of signaling and adhesion platforms. The biogenesis of endocytic vesicles is built on the precise spatial and temporal control of a protein machinery to undergo the remodeling of the plasma membrane.
The impaired function of several endocytic components is associated to human disorders such as cancer, myopathies or neurologic diseases. In addition, viruses and bacteria can exploit endocytic pathways as a means of entry into host cells, such as the HIV-1.


We apply quantitative approaches at the interface of cell biology, biophysics and nanotechnologies that we combine with the development of bottom up synthetic methods and cell-free systems to infer on the organization of cells at different length scales: from the single molecule to the cellular level.

 



Using minimal and in cellulo systems we could previously show that phosphoinositide clustering induced by the endocytic protein BIN1 could mediate the recruitment of its downstream phosphoinositide-binding partner dynamin on membranes (Picas L., et al. Nature Communications 2014). This mechanism was key to better understand why different BIN1 mutations in the autosomal form of centronuclar myopathies display a similar phenotype in dynamin binding. An important research line of the group is dedicated to elucidate if a similar mechanism is implicated in the coordination of trafficking events that rely on the local accessibility to phosphoinositide pools, such is the case of clathrin-mediated endocytosis (Picas L. et al. F1000 Research 2015).

We have also developed new methods to infer on the nanoscale organization of cellular membranes by combining Atomic Force Microscopy and Fluorescence Microscopy (Picas L. et al. ACS Nano 2013).









credits team picture: https://illustration4science.com/

Recent publications

2018
  • Tsai, F. C.;  Bertin, A.;  Bousquet, H.;  Manzi, J.;  Senju, Y.;  Tsai, M. C.;  Picas, L.;  Miserey-Lenkei, S.;  Lappalainen, P.;  Lemichez, E.;  Coudrier, E.; Bassereau, P., Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner. Elife 2018, 7. Link to paper.
  • De Franceschi, N.;  Miihkinen, M.;  Hamidi, H.;  Alanko, J.;  Mai, A.;  Picas, L.;  Guzman, C.;  Levy, D.;  Mattjus, P.;  Goult, B. T.;  Goud, B.; Ivaska, J., ProLIF - quantitative integrin protein-protein interactions and synergistic membrane effects on proteoliposomes. J Cell Sci 2018, 132 (4). Link to paper.
2016
  • Picas, L.; Gaits-Iacovoni, F.; Goud, B., The emerging role of phosphoinositide clustering in intracellular trafficking and signal transduction. F1000Res 2016, 5. Link to paper.
2015
  • Carretero-Genevrier, A.; Gich, M.; Picas, L.; Sanchez, C.; Rodriguez-Carvajal, J., Chiral habit selection on nanostructured epitaxial quartz films. Faraday Discuss 2015, 179, 227-33. Link to paper.
2014
  • Picas, L.; Viaud, J.; Schauer, K.; Vanni, S.; Hnia, K.; Fraisier, V.; Roux, A.; Bassereau, P.; Gaits-Iacovoni, F.; Payrastre, B.; Laporte, J.; Manneville, J. B.; Goud, B., BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin. Nat Commun 2014, 5, 5647. Link to paper.
2013
  • Rico, F.; Rigato, A.; Picas, L.; Scheuring, S., Mechanics of proteins with a focus on atomic force microscopy. J Nanobiotechnology 2013, 11 Suppl 1, S3. Link to paper.
  • Picas, L.; Rico, F.; Deforet, M.; Scheuring, S., Structural and mechanical heterogeneity of the erythrocyte membrane reveals hallmarks of membrane stability. ACS Nano 2013, 7 (2), 1054-63. Link to paper.
  • Rico, F.; Picas, L.; Colom, A.; Buzhynskyy, N.; Scheuring, S., The mechanics of membrane proteins is a signature of biological function. Soft Matter 2013, 9 (32), 7866-7873. Link to paper.
  • Carretero-Genevrier, A.; Gich, M.; Picas, L.; Gazquez, J.; Drisko, G. L.; Boissiere, C.; Grosso, D.; Rodriguez-Carvajal, J.; Sanchez, C., Soft-chemistry-based routes to epitaxial alpha-quartz thin films with tunable textures. Science 2013, 340 (6134), 827-31. Link to paper.

2012
  • Picas, L.; Suarez-Germa, C.; Montero, M. T.; Domenech, O.; Hernandez-Borrell, J., Miscibility behavior and nanostructure of monolayers of the main phospholipids of Escherichia coli inner membrane. Langmuir 2012, 28 (1), 701-6. Link to paper.
  • Picas, L.; Rico, F.; Scheuring, S., Direct measurement of the mechanical properties of lipid phases in supported bilayers. Biophys J 2012, 102 (1), L01-3. Link to paper.
  • Picas, L.; Milhiet, P. E.; Hernandez-Borrell, J., Atomic force microscopy: a versatile tool to probe the physical and chemical properties of supported membranes at the nanoscale. Chem Phys Lipids 2012, 165 (8), 845-60. Link to paper.

Team members


Past team members

  • Raïssa Rathar – M1 master student, Montpellier University.
  • Florian Hérault –  BSc (L3) student, Montpellier University.
  • Cyrielle Holuka –  M2 master student, Montpellier University.
  • Thibault Sansen –  M2 master student, Toulouse University.
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Local

National

International

Post-Doc, Thesis, Internship

We search for highly motivated candidates (biologists, biochemists, biophysicists, physicist) interested on interdisciplinary approaches applied to biological systems: from the single molecule to the cellular level.


NanoCytoLab News & Views

June 2019

Farewell party with French tapas! We will miss you Cyrielle and Thibault! All the best for your new adventures!



June 2019

This time the team has many things to celebrate and nothing better than a picnic under the sun! Happy birthday Cyrielle, Aïd Moubarak to all and a warm welcome to Charlotte for joining the team!



April 2019

The team will be presenting our most recent work at the Membrane Study Group (GEM) and Club Exocytose-Endocytose joint meeting on "Membrane Biophysics of Exo-Endocytosis: from Model Systems to Cells" at Cannes Mandelieu. Check out the exciting list of speakers!

 

Februrary 2019

We are very happy to welcome Cyrielle & Thibault, the new master’s students of the team.  Big thanks to Fatima’s mum for this exceptional couscous! It was a great picnic day with friends and collaborators!

 


November 2018

This year Christmas came earlier… with the new AFM of the team! Scanning days are back!

 

August 2018

Farewell Raissa and Florian! We very much enjoyed hosting you in the team! We wish you all the best!

Team leader

Laura Picas

ATIP-Avenir Team Leader
Tenure researcher CRCN CNRS
En savoir +


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At a glance



Welcome to the NanoCytoLab!

We are a young and multidisciplinary team that has been established at IRIM in Septembre 2017 thanks to the support of the ATIP-Avenir program.

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Funding

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Contact us


     
   

   

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IRIM
Institut de Recherche en Infectiologie de Montpellier
UMR 9004 - CNRS / UM
1919 route de Mende - 34293 Montpellier cedex 5
FRANCE

 

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